Shake Well! Bacillus spores settle naturally. Use once per week, or as frequently as every watering during the entire plant growth cycle. May be used up to and on day of harvest. Use mixed nutrient solutions within 24 hours for best results. For use with hydroponic, soilless, and soil-based medias. Can be mixed with aqueous nutrient solutions and/or applied directly to plant roots at the recommended rates. APPLICATION NOTES Applying Tribus• Original early in the plant's life cycle encourages rapid establishment of Bacillus colonies on and near plant roots. Continued applications of Tribus• Original as a supplement to normal fertilization programs helps to maintain high populations of beneficial bacteria in the root zone, promoting vigorous plant growth from seedling and clone phases through harvest.